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College of Arts and Sciences


Chemistry and Biochemistry Seminar Series: Dr. Stephen Sligar

Friday, September 15, 2017 - 4:00pm

Location: Jones PSC, Room 006

Speaker: Dr. Stephen Sligar, School of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign

Topic: Revealing the Structure and Function of Membrane Proteins Through Nanotechnology

Abstract: Membrane proteins are involved in numerous vital biological processes, including transport, signal transduction and the enzymes in a variety of metabolic pathways. Integral membrane proteins account for up to 30% of the human proteome and make up more than half of all currently marketed therapeutic targets. Unfortunately, membrane proteins are inherently recalcitrant to study using the normal toolkit available to scientists, and one is most often left with the challenge of finding inhibitors, activators and specific antibodies using a denatured or detergent solubilized aggregate. Often, since membrane proteins are inherently insoluble and prone to aggregation and oligomerization. in solution, the active state of interest is obscured. The Nanodisc platform circumvents these challenges by providing a self-assembled system that renders typically insoluble, yet biologically and pharmacologically significant, targets such as receptors, transporters, enzymes, and viral antigens soluble in aqueous media. Because Nanodisc constructs provide a native-like bilayer environment that maintain a target’s functional activity, they are a versatile tool in the study of membrane proteins such as ion channels, GPCRs, cytochrome P450s, blood coagulation factors, various toxins and viral entities as well as a plethora of pharmaceutical targets. In addition to the opportunities in drug discovery, Nanodiscs provide a nanometer scale vehicle for the in vivo delivery of amphipathic drugs, therapeutic lipids, tethered nucleic acids, imaging agents and active protein complexes. In my presentation, I will focus on recent uses of the Nanodisc technology in seeking mechanistic understanding of metalloprotein oxygenases, integrin and KRas4b signaling and Alzheimer’s disease intervention

 

Sponsored by the College of Arts and Sciences and the Department of Chemistry and Biochemistry

 


PDF icon Chemistry Lecture, September 15, 2017